I want to download and select specified No. of introns sequence from UCSC. eg. introns:2,7,8,12,16,19,20 of gene:BRCA1 from FoundationOneCDx.

Here is my steps.

1. download all introns sequence from UCSC.

   Tools -> Table Browser -> clade:Mammal;gemome:Human;assemble:hg19;grop:Genes and Gene Predictions;track:NCBI RefSeq;table:RefSeq All -> paste list: BRCA1(for example) -> get output -> Sequence Retrieval Region Options:Introns(One FASTA record per region (exon, intron, etc.) with...) -> get sequence:fasta file

2. select specified introns.

The fasta file includes 130 Introns sequence from different NM on "strand=-", and some introns have the same chromosome coodinates. Here is my thought to get specified introns:

extract all introns chromosome coordinate -> remove duplicated ones -> sort by chromosome coordinate

  The filter result:
  1 range=chr17:41197800-41199679
  2 range=chr17:41199701-41201157
  3 range=chr17:41199701-41203099
  4 range=chr17:41201192-41203099
  5 range=chr17:41203115-41209088
  6 range=chr17:41209133-41215369
  7 range=chr17:41215371-41215910
  8 range=chr17:41215949-41219644
  9 range=chr17:41219693-41222964
 10 range=chr17:41223236-41226367
 11 range=chr17:41226519-41228524
 12 range=chr17:41228609-41231370
 13 range=chr17:41228609-41234440
 14 range=chr17:41228612-41234440
 15 range=chr17:41231397-41234440
 16 range=chr17:41234573-41242980
 17 range=chr17:41243030-41243471
 18 range=chr17:41243030-41246780
 19 range=chr17:41246858-41247882
 20 range=chr17:41247920-41249280
 21 range=chr17:41249287-41251811
 22 range=chr17:41251875-41256158
 23 range=chr17:41251878-41256158
 24 range=chr17:41256259-41256904
 25 range=chr17:41256954-41258492
 26 range=chr17:41256954-41258514
 27 range=chr17:41258531-41267762
 28 range=chr17:41258531-41276053
 29 range=chr17:41267777-41276053
 30 range=chr17:41276113-41277218
 31 range=chr17:41276113-41277307
 32 range=chr17:41276113-41277313

My question is that:

(1) line 2,7,8,12,16,19,20 is the Introns 2,7,8,12,16,19,20 of BRCA1

(2) is there difference between strand=+ and strand=- to get introns sequence

(3) how do deal with overlap chromosome coordinate from above result? eg line 2,3,4

Maybe my thought and step are totally wrong, any help will be appreciated.