Off topic:New to Whole Exome Sequencing and Manhattan Plot and I need to understand how to compare patients' data with healthy control and find p value and then plotting Manhattan plot.

Question

[jaybee]

Hi all,

I am a 1st year MS student and I am required to compare the data I have with the healthy controls, then find p value and pot the Manhattan plot to find out which mutation is abundant for the condition.

The data I have been given has rsid and gene names, EXAC all and EXAC 1000

I want to know:

how do I compare the entire data I have with the data of the healthy control?

How do I then find the p value?

How do I then plot Manhattan Plot?

Please guide me.