PacBio consensus assembly quality value
Hello
I have sequenced a BAC clone with PacBio RSII To make the assembly I used Facon through pbbioconda and for polishing I used quiver
To have an estimation of the consensus quality I remap the original bam reads file against the consensus fasta file
How to estimate a mean quality value, in other world, a consensus Phred score for the base calls of the consensus ... :-)
Thank you very much in advance Philippe
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