We are planning to do a crispr library for several experiments. I would to ask the group here what is the best way to know which genes to take into the library.
Let's say we would like to analyze the RNA Polymerase II activity. Would it be enough to look up all the gene in the related GO category or functional annotations and create the sgRNA-library with these genes? E.g. GO:0001055 (RNA polymerase II activity) orGO:0006366 (transcription by RNA polymerase II) etc.
Or are there any special pre-prepared library one can order/ use?
Do one need to pay attention to any specific restrictions or limitations when such a library is created (ordered)?
I would appreciate any suggestions. thanks