Got it! Thanks a lot!
In the galaxy, when I use RNA STAR to map the reads, it requests the "Gene model (gff3,gtf) file for splice junctions", and then when I use the Htseq or featureCounts, it requests the "Gene annotation file or GFF file".
I am wondering whether for this I can use the same GTF file? or one is specific for splice junctions, another is specific for gene names?
Thank you so much!
1 answer
Yes, in theory you can swap those files. You just need to make sure they are both correctly formatted and that they contain the same 'naming' : that eg. gene_id is consistently used to denote the genes (and thus not gene_id in file A and parent_id in file B), or adapt your htseq count cmd accordingly.
Those two file types are nothing specifically for splice sites and/or names. They are simply alternatives from each other, they both contain gene annotations in their respected format
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