SRA submission Error: Command based

Question

[suren04]

Hi All, I am using command scripts for submitting my fastq.gz files to NCBI. However, I got this error message repeatedly. What could be the possible reason while submitting the fastq.gz file to NCBI SRA database?

ERROR is:
ftp> mput *
local: 3-1a_S25_L005_R1_001.fastq.gz remote: 3-1a_S25_L005_R1_001.fastq.gz
200 PORT command successful
150 Opening BINARY mode data connection for 3-1a_S25_L005_R1_001.fastq.gz
netout: Broken pipe
421 Service not available, remote server has closed connection

Thanks

[GenoMax]

It is possible that you are behind a firewall that isn't allowing the incoming reverse connection from the ftp server to your client. Check with your IT folks to make sure ftp is allowed on this system.