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This 5X binding buffer where participates?

I saw 5X bingind buffer maded by;

  • 100mM HEPES, pH 7.6
  • 5mM EDTA
  • 50mM ammonium sulfate
  • 5mM DTT
  • 1% Tween-20
  • 150mM potassium chloride

which reaction helps this buffer? I don't consider to this buffer participates in which experiment by name. Although this buffer have another name but they writes just '5X bonding buffer'..

experiment

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