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How to do postivie selection using VCF data ?

what is the current way to do positive selection using VCF data from 20 different horse species (with same reference genome) I would like to construct phylogeny and looking for non-synonyms snps shared among all these groups. And, how many are common and is their any unique variants among these specific groups which are not shared?

I'm looking for out-group comparison to reconstruct phylogeny ?

suggestions please!

snp genome

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