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Error: Could not find Bowtie 2 index files (splice-mismatch.*.bt2)

hi i am running tophat2 and bowtie2 , these are my commands and i couldn't get the result:

tophat2 -p 16 --keep-fasta-order -o ./tophat_out_${NAME}\
 --read-mismatches 4\
 --read-gap-length 4\
 --read-edit-dist 4\
 --read-realign-edit-dist 0\
 --min-anchor 4\
 -- splice-mismatch 1\
 --GTF gencode.v19.annotation.gtf\
 --transcriptome-index= transcriptome/\
 genome/Homo_sapiens.bowtie_index\
 trimmed_cut1SRR5481725.fastq trimmed_cut2SRR5481725.fastq

and that's the error massage that i face:

[2018-12-05 09:23:33] Checking for Bowtie index files (genome)..
Error: Could not find Bowtie 2 index files (splice-mismatch.*.bt2)
rna-seq software error sequencing next-gen tophat2

Please do not use Tophat anymore, the author himself said it's outdated

HISAT2 and STAR are both splice aware solution

Hmmm the command you show doesn't match with the error you get?

1 answer

You have a space in -- splice-mismatch. As mentioned in the comment, you should not be using tophat for anything.

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