Entering edit mode
5.4 years ago
swanti.gupta95
•
0
I am trying to use BiBiServ-RNAHybrid but am encountering certain problems.KIndly help me asap.
I wish to obtain the following results using RNAHybrid (Objectives) -
A) I wish to find the minimum free energies of the miRNA interacting with the 3'UTE/5'UTR or CDS of the gene
(eg.APOE )
B)Structure
c)Alignment details
The problem I am having-
a)If gene seq ( FASTA format )or mRNA seq (FASTA format ) is taken as target seq.
(how is mRNA FASTA obtained if so)
b) what to fill in the options -
helix constraint from
helix constrain to
max bulge loop length
max internal bulge loop length
Would someone PLEASE explain me with an example like >ENSG00000130203|ENST00000252486|.It is APOE transcript and miRNA- hsa-mir-650
