Hi Biostars,

I am trying to design primers. For this I have multiple sequences in a fasta file and I have found this program that give kind of nice visualisation:

https://www.ncbi.nlm.nih.gov/tools/primer-blast/

1) However I cannot paste multiples sequences .. Is it normal or I am doing something wrong ?? I got this error:

Exception error: Multiple templates are currently not supported! .

2) A command line version does'nt exist ?

3) If no, do you have any recommendation for other programs that take multiple input and/or can be run by command line ?

Thanks a lot.