Hi all,
I'm pretty new with kallisto and sleuth, but thanks to the online information I'm getting result of my RNA-seq analysis. I'm following this pipeline https://pachterlab.github.io/sleuth_walkthroughs/trapnell/analysis.html to see different gene expresion.
Now I'm triying to run "plot_bootstrap function"
In the example of the web say:
plot_bootstrap(so, "ENST00000263734", units = "est_counts", color_by = "condition")
In my case the most expressed transcript is called TRINITY_DN3505_c0_g1_i5 and my factor is "seasons". Then I tried with that:
plot_bootstrap(so, "TRINITY_DN3505_c0_g1_i5", units = "est_counts", color_by = "season")
But I got the error:
Error in obj$bs_quants[[1]] : subscript out of bounds
Can you help me with the meaning of this error?
I was reading other post about this error, and it appears to mean that you are calling to something that doesn't exits... but I don't undersand, because in my case the name of the transcript is correct and also the name of the factor... both exist. So... I don't know.
Any suggestion could be helpful.
Member to mark your answer as the correct solution.