Entering edit mode
5.5 years ago
owenz
▴
20
I installed Kraken on Linux and was about to build the bacterial db. Unfortunately, the Jellyfish version installed was 2.2.8 which is not compatible with Kraken.
I uninstalled this version and tried to install the necessary 1.1.11 version through apt. Unfortunately it was not retrieved.
I download the tar.gz and tried to change the PATH variable but when I tried to enter the Kraken build command, it was still not effecting.
Could this also be a problem with Kraken and the threads required? What should be the ideal thread count?
Thanks in advance
The problem now seems to be to limit hash size on a 32gb Ram PC. What should be ideal?
Find other hardware with more RAM? There is really no substitute for lack of adequate RAM.
Both Kraken and Kraken2 provide a "MiniKraken" pre-built database, including bacterial, archaeal, and viral genomes:
https://www.ccb.jhu.edu/software/kraken/dl/minikraken_20171019_8GB.tgz
https://www.ccb.jhu.edu/software/kraken2/dl/minikraken2_v1_8GB.tgz