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-bash: ./HeatMap.R: cannot execute binary file. Why is this?

Hello everyone, I am trying to run an R script to generate a heat-map but I keep getting the following error. I have no idea what is wrong with my input file. My input looks like this:

      male   female   juvenile   egg   blastula
 Aub  13.3   34.1     2323       321   3231
 piwi   23     34        34           54      534 
 Ago  35     65         34           34      56
 Ago2 0       0           0            0         0

-bash: ./HeatMap.R: cannot execute binary file

my R script look like this.

#!/usr/bin/env Rscript
library("gplots")
Data <- read.csv("input.txt", sep = "\t", header = TRUE)
DataMatrix <- data.matrix(Data)
rownames(DataMatrix) <- c("Aub", "piwi", "Ago", "Ago2")
colnames(DataMatrix) <- c("male", "female", "juvenile", "egg", "blastula")
png('ZscorePlot.png')
DataMap <- heatmap.2(DataMatrix, trace="none", dendrogram ='none', Rowv = FALSE, Colv = FALSE, col = colorRamps::matlab.like2, key=TRUE, keysize=1.0, xlab = "Stage in Development", ylab = "RNAifactor")
dev.off()

Is there something wrong? by the way, this is not my original data, just a sample to show you how it looks like. thanks.

heatmapscript mapping rnaseq rpm
• 5,201 views • link
written by jaqx008 jaqx008 
rownames(DataMatrix) <- c("Aub", "piwi", Ago")
----------------------------------------^

You're missing a double quote.

• 1 views • link
written by Ram Ram

Thats me typing on here. its all good in my R script. I corrected that on the post. In my script its all good. But error persists

• 1 views • link
written by jaqx008 jaqx008

Why is your R script binary? Have you tried Rscripts myscripts.R? The cannot execute binary file typically has something with incompatible architectures.

• 1 views • link
written by Eric Lim Eric Lim

to be honest I am not sure what you mean.

• 1 views • link
written by jaqx008 jaqx008

What does it return with the command file HeatMap.R?

• 1 views • link
written by Eric Lim Eric Lim

it returns this, 

-bash: ./HeatMap.R: cannot execute binary file
• 1 views • link
written by jaqx008 jaqx008

You ran

./Heatmap.R

Eric asked you to run

file Heatmap.R
• 1 views • link
written by Ram Ram

file Heatmap.R gave the following and didnt generate anything in folder

HeatMap.R: data

I apologies for late response. I am limited to few post per certain hours.

• 1 views • link
written by jaqx008 jaqx008

The file utility tells you what kind of file Heatmap.R is, it does nothing else - that's expected. Think of it as a sanity check on the file. It is strange that you get data.

• 1 views • link
written by Ram Ram

I think it should be Rscript file.R, not Rscripts.

• 1 views • link
written by Ram Ram 
Rscript Heatmap.R

gave this

Error: unexpected input in "book" Execution halted

sorry for late response, I am limited to ew posts

• 1 views • link
written by jaqx008 jaqx008

Of course. Now everybody knows I hardly run any R script. :D

• 1 views • link
written by Eric Lim Eric Lim

I tried to execute the commands one at a time on command line and upon reaching 

> rownames(DataMatrix) <- c("Bfl-Piwi-like1","Bfl-Piwi-like3","Bfl-Piwi-like2","Bfl-Piwi-like5","Bfl-Piwi-like4","Bfl-Ago1","Bfl-Ago-like","Bfl-Rdrp4","Bfl-Rdrp1","Bfl-Rdrp2","Bfl-Rdrp3","Bfl-Rdrp5","Bfl-Rdrp6","Bfl-Dro","Bfl-Dcr")

it gave the error

Error in dimnames(x) <- dn : 
  length of 'dimnames' [1] not equal to array extent
• 1 views • link
written by jaqx008 jaqx008

Your R script has errors then. Please fix them before trying to execute the script from the command line.

• 1 views • link
written by Ram Ram

You shouldn't execute an Rscript with bash.

Please follow up on all your previous posts:

If an answer was helpful you should upvote it, if the answer resolved your question you should mark it as accepted.
Upvote|Bookmark|Accept

• 1 views • link
written by WouterDeCoster WouterDeCoster

You need to run your file as:

$ Rscript HeatMap.R

You cannot run Rscripts with the R binary or with bash. Your shebang line should be taking care of this though.

What do you get for which -a Rscript?

• 1 views • link
written by Joe Joe 
which -a Rscript

/usr/local/bin/Rscript

• 1 views • link
written by jaqx008 jaqx008

2 answers

Column names are 5 and the number of columns are 6. The issue can be addressed by setting the first column as row names.

#!/usr/bin/env Rscript
library("gplots")
Data <- read.csv("input.tsv", sep = "\t", header = TRUE, row.names = 1) #first col as rownames
DataMatrix <- data.matrix(Data)
#rownames(DataMatrix) <- c("Aub", "piwi", "Ago", "Ago2") #Already set by row.names = 1
#colnames(DataMatrix) <- c("male", "female", "juvenile", "egg", "blastula") #Already set by header=TRUE
png('ZscorePlot.png')
DataMap <- heatmap.2(DataMatrix, trace="none", dendrogram ='none', Rowv = FALSE, Colv = FALSE, col = colorRamps::matlab.like2, key=TRUE, keysize=1.0, xlab = "Stage in Development", ylab = "RNAifactor")
dev.off()

Run using the Rscript command also make sure the data table is properly formatted.

input.tsv

        male    female  juvenile        egg     blastula
Aub     13.3    34.1    2323    321     3231
piwi    23      34      34      54      534
Ago     35      65      34      34      56
Ago2    0       0       0       0       0
• 1 views • link
written by Arup Ghosh Arup Ghosh

this is the error after i run it

Attaching package: ‘gplots’

The following object is masked from ‘package:stats’:

    lowess

Error in make.names(col.names, unique = TRUE) : 
  invalid multibyte string at '<fe><ff>'
Calls: read.csv -> read.table -> make.names
In addition: Warning messages:
1: In read.table(file = file, header = header, sep = sep, quote = quote,  :
  line 1 appears to contain embedded nulls
2: In read.table(file = file, header = header, sep = sep, quote = quote,  :
  line 2 appears to contain embedded nulls
3: In read.table(file = file, header = header, sep = sep, quote = quote,  :
  line 3 appears to contain embedded nulls
4: In read.table(file = file, header = header, sep = sep, quote = quote,  :
  line 4 appears to contain embedded nulls
5: In read.table(file = file, header = header, sep = sep, quote = quote,  :
  line 5 appears to contain embedded nulls
Execution halted
• 1 views • link
written by jaqx008 jaqx008

Hi,

I've updated the code there is no need to set rownames and colnames separately.Heatmap

• 1 views • link
written by Arup Ghosh Arup Ghosh

I realized my Text file had a tab in the first row. removing the tab makes the code run without a problem. Thanks for all your help. I appreciate it.

• 1 views • link
written by jaqx008 jaqx008

I added code markup to your post for increased readability. You can do this by selecting the text and clicking the 101010 button. When you compose or edit a post that button is in your toolbar, see image below:

101010 Button

• 1 views • link
written by WouterDeCoster WouterDeCoster

If an answer was helpful you should upvote it, if the answer resolved your question you should mark it as accepted.
Upvote|Bookmark|Accept

• 1 views • link
written by WouterDeCoster WouterDeCoster

new text file that made the code work looks like this

name  male    female  juvenile        egg     blastula
Aub     13.3    34.1    2323    321     3231
piwi    23      34      34      54      534
Ago     35      65      34      34      56
Ago2    0       0       0       0       0
• 1 views • link
written by jaqx008 jaqx008

Sorry, why is this an answer? Please use comments unless you're answering the top level question.

• 1 views • link
written by Ram Ram

I added correction to my initial text file. tab replaced with name.

• 1 views • link
written by jaqx008 jaqx008

why is this an answer?

That does not answer the above question. You should have edited your post and added this info there, not added it as an answer. Please edit and add now, and then delete this answer.

• 1 views • link
written by Ram Ram

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