Per base sequence content
Good afternoon!
I'd like some help in a problem I have been facing in trimming my sequenced genome. Even after using the value of 3 for the LEADING and TRAILING, the FASTQC webpage keeps showing a error message. Is there anything that can be done to solve this problem?
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vitorgomesbio •
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Please use these instructions to add images in-line: How to add images to a Biostars post
Trying to open the link you posted gives a 403 error. And the way you linked the image here, regular users can't even see the link. Please fix these issues, otherwise it will be difficult to help you.
One of the most common causes for warnings / errors for the per-base sequence content diagnostic is that the random priming step in library preparation is not truly random:
Positional sequence bias in random primed libraries
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