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R program for normalization of RNA seq counts data with Edge R or Deseq

Can anybody guide me about normalization of RNA seq( Htseq-counts data ) using edgeR or Deseq2,i need help about R program for normalization of RNA seq using EdgeR.

rna-seq r sequencing

1 answer

Normalization of the data is managed by the packages mentioned. You need to feed them raw count data.

problem is i don't know the exact way of using these packages and feeding data to them

Workflow for DESeq2 is described in detail here. For edgeR you can find similar information here.

i have ht-seq-count input in the form of excel file(CSV) but in these link i am not able to find how to normalize such kind of files..

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