problem is i don't know the exact way of using these packages and feeding data to them
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Can anybody guide me about normalization of RNA seq( Htseq-counts data ) using edgeR or Deseq2,i need help about R program for normalization of RNA seq using EdgeR.
Normalization of the data is managed by the packages mentioned. You need to feed them raw count data.
problem is i don't know the exact way of using these packages and feeding data to them
Workflow for DESeq2 is described in detail here. For edgeR you can find similar information here.
In addition, focus on the section in the DESeq2 manual, htseq-count input.
i have ht-seq-count input in the form of excel file(CSV) but in these link i am not able to find how to normalize such kind of files..
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