Hello, I have raw read counts from various stress conditions (4 types) from the same RNA-seq platform and tissue. I had previously determined the DEGs from the individual stresses separately and also extracted their normalized expression matrix for each of the stress conditions .
If I wish to perform a consensus module analysis using the WGCNA frame-work, do I need to perform 'vst' normalization in DeSeq2 across all the conditions ? Any leads to go about it will be very useful. Thanks