SmallRNA NGS - high unmapped reads - is this normal?
Hi, we just recently started performing NGS for small RNA in exosomes (human). We are new to RNA processing using ion torrent. We are getting only 20-25% usable reads and only 10-15% mapped reads with only 1-3 detected miRNA (quantity of 10 or more). Is this normal? What is the expected range? Thank you for your help.
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