shift direction in ATAC/DNase data?
1
0
Entering edit mode
5.6 years ago
star ▴ 350

I have two set of data about ATAC-seq and DNase-seq and I like to perform peak calling using MACS2, but I found that there are some differences in shift size (plus or minus direction). I like to know which one is better for either ATAC-seq or DNase-seq (e.g. --nomodel --shift -100 --extsize 200 or --nomodel --shift 100 --extsize 200). is it right that because most accessible regions in ATAC-seq and DNase-seq located in 5' region so using --shift -100 is recommended?

ChIP-Seq MACS2 ATAC-seq DNase-seq • 1.8k views
ADD COMMENT

Login before adding your answer.

Traffic: 2013 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6