Hi all,

I'm trying to run Trinity (2.6.6.) to analyze a metatranscriptome, but it is giving me the following errors:

Error, pairs.K25.stats is empty.  Be sure to check your fastq reads and ensure that the read names are identical except for the /1 or /2 designation. at /fs/project/PAS1117/modules/trinity/2.6.6/util/insilico_read_normalization.pl line 921.
CMD finished (1209 seconds)
Error, cmd: /fs/project/PAS1117/modules/trinity/2.6.6/util/insilico_read_normalization.pl --seqType fq --JM 112G  --max_cov 50 --min_cov 1 --CPU 28 --output /fs/project/PAS1117/GuiDH/Trinity_tests/trinity_out_dir/insilico_read_normalization   --max_pct_stdev 10000  --left /fs/project/PAS1117/Tara_Euk/upwelling-v2/ERR1719296_1.fastq.gz --right /fs/project/PAS1117/Tara_Euk/upwelling-v2/ERR1719296_2.fastq.gz --pairs_together --PARALLEL_STATS   **died with ret 512 at /fs/project/PAS1117/modules/trinity/2.6.6/Trinity line 2581.
        main::process_cmd('/fs/project/PAS1117/modules/trinity/2.6.6/util/insilico_read_...') called at /fs/project/PAS1117/modules/trinity/2.6.6/Trinity line 3127
        main::normalize('/fs/project/PAS1117/GuiDH/Trinity_tests/trinity_out_dir/insil...', 50, 'ARRAY(0x2735d58)', 'ARRAY(0x277bb80)') called at /fs/project/PAS1117/modules/trinity/2.6.6/Trinity line 3074

Could someone check if there is something wrong with the batch file sent? (pasted here below)

#PBS -N trinity_test3
#PBS -l walltime=40:00:00
#PBS -l nodes=1:ppn=48
#PBS -j oe
#PBS -m abe
#PBS -A PAA0034
set -x
cd $PBS_O_WORKDIR
module use /fs/project/PAS1117/modulefiles
module load samtools/1.3.1
module load bowtie2/2.2.9
module load jellyfish/2.2.9
module load salmon/0.9.1
module load trinity/2.6.6
Trinity --seqType fq --left /fs/project/PAS1117/Tara_Euk/upwelling-v2/ERR1719296_1.fastq.gz --right /fs/project/PAS1117/Tara_Euk/upwelling-v2/ERR1719296_2.fastq.gz --CPU 28 --max_memory 112G
echo $PBS_JOBID
echo $PBS-JOBNAME

Thank you!