monocle clustering cells without marker genes error
I am using monocle to analyse my gene_counts matrix.
I am trying to use function :clustering cells without marker genes. And I am following the guideline on the website.
My code is:
HSMM <- reduceDimension(HSMM, max_components = 2, num_dim = 6, reduction_method = 'tSNE', verbose = T)
The error is :
Remove noise by PCA ...
Reduce dimension by tSNE ...
Error in Rtsne.default(as.matrix(topDim_pca), dims = max_components, pca = F, :
Remove duplicates before running TSNE.
What should I do about it? I appreciate your help.
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