software for Insilico Pcr
Hello Friends, I hope u all are doing good. I have desgined primers using primer_3_core and i have obtained 70,000 primer pairs, now i want to do the insilico pcr of these primers, can anybody told me any good software for insilico pcr, i am using ubuntu operating system. Any command based application would me much more appriciable, or any script.
Thanks in advace.
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moohit21 •
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moohit21 •
1 answer
Try the University of California, Santa Cruz's (UCSC's) in silico PCR tool: https://genome.ucsc.edu/cgi-bin/hgPcr
Kevin
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Kevin Blighe
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@kevin.. Thanks for your answer, but how i am going to put 70,000 primer pairs in it, it is showing the space for only 1 primer pair
See at the bottom of the page to which I have linked you:
Jim'll fix it for you.
It is also possible to programmatically engage with web-forums via, for example, shell scripts, but I would not recommend it in this case as it may break UCSC's 'good use' policy.