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Abundance of thymine base in the neighborhood of breast cancer mutations

I have been analyzing breast cancer mutations from COSMIC. One of the observations I made was, for cancer-causing mutations, there is an abundance of thymine nucleotides immediately adjacent(left side) to the mutation site. How can I validate this result? Can anyone point me to some published resource/tool that can help me prove the over-representation of certain bases flanking the mutation sites?

genome snp sequence
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written by Gene_MMP8 Gene_MMP8

That's pretty granular information. Are you sure it's not confirmation bias? I think you're going to need a custom script for that.

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written by Ram Ram

Can you please elaborate? I have already tested that the abundance is statistically significant with a p value <10e-11. Are you implying on making a custom script that tests whether the abundance is statistically relevant?

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written by Gene_MMP8 Gene_MMP8

I was indeed suggesting that you test its statistical significance, which you've already done.

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written by Ram Ram

Perhaps you need to do this: Take a) different cancer dataset(s) and/or b) a non-cancer dataset(s). Repeat the analysis. See if the correlation does not exist in those. Especially if you are looking to correlate it specifically to breast cancer.

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written by GenoMax GenoMax

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