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5.7 years ago
kylin.qhl
•
0
Hi,
I'm new to WGBS analysis. I have the human WGBS data from sequencing company along with a report. Now I'm trying to follow the report and replicating every step, but I got a problem with the DMR annotation. The company used Bismark to call the methylation sites and used DSS package in R to do DMR analysis. But I don't have an idea about which package they used to do the annotation and got the table below.
This is the DMR result from the company:
Thanks very much for the further help!
Did you pay for the analysis? Demand this information, in details, from the company. How are you going to interpret or publish if you don't know the details?
This data is from one of the collaborators' lab. They want me to do the analysis again instead of using the result from the company. You are right, I should write an email to the company for the information. I'm just curious about how to annotate the same sequence with different region and merge the two tables.
I understand the "merge the two tables part", but not the "annotate the same sequence with different region". What do you mean?
For example, chr1 29297:30663, the intro, promoter, exon etc can be identified separately and then for this single sequence there would be multiple rows in the final table. I'm not sure if this is clear? Thanks.