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RSEM log 2(norm count +1)

hello! I was wondering how one could interpret RSEM log2(norm_count +1) difference between Control = 1 and Treatment = 8 for a given gene. Is it the difference between Control = 2 (2^1) and the Treatment = 256 (2^8)?

Thanks!

Saumya

rna-seq
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updated by Biostar Biostar • written by saumram • saumram

1 answer

That sounds about right, you'd need to subtract the pseudocount though just to be 100% accurate. y = log2(x+1) => x = (2 ^ y) - 1

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written by Ram Ram

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