How to characterise a protein "enzyme", and find evolutionary divergence into two distinct pathways?

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5.8 years ago

arpankbasak ▴ 10

Hi, I was wondering if any one can tell me, how I can characterise a protein divergence evolution significant in metabolic pathway. I'll be using rDNA technology to express the possible genes of these "proteins" or "enzymes" to be more specific to express them in a vector and then infect them on plants.

I am asking for a short one-liner how to proceed and what parameters should I emphasise the most..?

Any explanation will be of great help.

Proteins Enzymes Protocol Pathway • 1.2k views

ADD COMMENT • link 5.8 years ago by arpankbasak ▴ 10

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asking for a short one-liner

I hope you don't mean code?

ADD REPLY • link 5.8 years ago by Joe 21k

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No, I meant simple steps

like steps represented with ">" or "|"

Example:

Dna_Isolation > Quantification > PCR > Library_preparation ...

ADD REPLY • link 5.8 years ago by arpankbasak ▴ 10

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This a general review, it may clear some points.

Divergence and Convergence in Enzyme Evolution

Michael Y. Galperin and Eugene V. Koonin

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3249071/

ADD REPLY • link 5.8 years ago by natasha.sernova ★ 4.0k

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Thanks, this article provides a good start.

ADD REPLY • link 5.8 years ago by arpankbasak ▴ 10

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