I'm looking for a convenient tool, to demultiplex my Illumina PE data. Particularly to extract pairs with a certain sequence in the forward read and other certain sequence in the reverse one. Could you advise me please? For example: Initially, we have two fastq files with forward and reverse reads
Forvard reads sequences:
Reverse reads sequences:
So, i'd like to extract for futher analysis only pair
Since in the forward read is AGTCCGTATATGCCGAG tag and there is GAGATGGACTACTCAC tag in the reverse read. Now i need only 100% match.