Hi all!

I am working with 2 pools of full genome sequencing data. I currently have a VCF file for each pool and one VCF with the two combined (bcftools merge). Now I want to calculate the Fst for each SNP with vcftools, but I am a bit lost at this point. It says I have to provide one file for each population with a list of all individuals within one of those populations. I have used "bcftools query -l file.vcf" to see the samples in the merged files and it lists the correct two. When I use these two sample names in the .txt files for the Fst script it renders me an Fst saying "NA" at each locus... What am I doing wrong? Also, the "ID" column in my VCF is "."

Thanks!