Homer (http://homer.ucsd.edu/homer/ngs/annotation.html) can be used to do ChIP-seq regions annotation. I do not use homer to do the whole ChIP-seq analysis. So i do not get homer peak files. I use macs2 to do peak calling and get bed file. But the file did not contain strand column. So I want know whether this strand is necessary.Once i use the bed file from macs2 and use Homer to do region annotation. The output fie will include the strand colume, but all the strands show "+". Does this right? Thanks.