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Generate the sequence dictionary

I am trying to generate sequence directory by running the following command in picard tools.

java -jar picard.jar CreateSequenceDictionary REFERENCE=new_hg38.fa OUTPUT=reference.dict

But it returns with this error.

Error: Unable to access jarfile picard.jar

Actually I am new to this any help would be appreciated,

alignment software error picard

Hi jahanzaib119311

Please use the formatting bar (especially the code option) to present your post better. I've done it for you this time.
code_formatting

Thank you!

1 answer

This can be solved by qualifying the path to your picard.jar file. For example, if you have downloaded picard.jar to the bin subdirectory of your home directory, you would change your command line to. java -jar $HOME/bin/picard.jar

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