Dear Dr. Warner
Thanks for your comment. I have another problem. As you know for preparing output file for gene ontology analysis in WGCNA, I need GeneAnnotation.csv file for my Gene Expression profile. My Gene Expression data set is RNA-seq data from The Genome Cancer Atlas (TCGA). For that purpose I downloaded "gencode.v22.genes.csv" but the attributes name of "gencode.v22.genes" is not similar to GeneAnnotaion.csv file in Tutorials for the WGCNA package.
I don't know how should I handle Annotation file for my dataset?
And my second problem is that below code is based on MicroArray and my data set is RNA-seq.
# Read in the probe annotation
GeneAnnotation=read.csv(file="GeneAnnotation.csv")
# Match probes in the data set to those of the annotation file
probes = names(datExprFemale)
probes2annot = match(probes,GeneAnnotation$substanceBXH)
# data frame with gene significances (cor with the traits)
datGS.Traits=data.frame(cor(datExprFemale,datTraits,use="p"))
names(datGS.Traits)=paste("cor",names(datGS.Traits),sep=".")
datOutput=data.frame(ProbeID=names(datExprFemale),
GeneAnnotation[probes2annot,],moduleColorsFemale,datKME,datGS.Traits)
# save the results in a comma delimited file
write.table(datOutput,"FemaleMouseResults.csv",row.names=F,sep=",")
How can I customize that code for RNA-seq?
I appreciate if you share your comment with me.