In RSAT retrieve_Ensembl_seq do FASTA sequences of genes on reverse strands take this into account?
I am retrieving some orthologous promoter regions relative to the mRNA, some of the reference genes are on the reverse strand. What I want to know is if the retrieved FASTA sequence is of the reverse strand and is it flipped 3' to 5' so that the gene code can be read left to right, or if the default is to extract the forward strand 5' to 3'.
Or do I need to get the reverse complement, or just reverse the sequence?
• 1,786 views
•
link
written
by
Monika515 •
0 answers
No answers yet.
Log in to answer this question.
More posts like this
-
Understanding vg filter nodes output in terms of forward and reverse compliment strand origin
written by cassiwatt •
-
Distinguish between forward strand, plus strand, sense strand
written by tien •
-
infer the orientation of z score by using the harmonisation codes
written by celia •
-
Getting the mRNA sequence from genome annotation
written by colin.kern
-
Parsing out very similar sequences from paired amplicon sequencing
written by geo •
-
position specific sequence retrieval from whole genome sequence
written by psiwach29 •
-
Aligning TF binding site to target gene method
written by bharata1803
-
Paired-end reads - what is what?
written by darxsys
-
Poly(A) Signal Prediction And Forward/Reverse Strand Conventions
written by Lídia •
-
Forward And Reverse Strand Conventions
written by Andrea_Bio •
