Error while running MEGAX
I am running the latest version of MEGA i.e MEGAX. I am trying to run the command megacc but it is throwing an error.
My sequences.fas file contains the trimmed multiple sequence alignment (MSA) of 16S rRNA sequences of 1403 organisms in a fasta format. This file was generated after running MUSCLE (produces an MSA) and BMGE ( it performs entropy based trimming on the MSA).
The file "analysisFile.mao" was generated using the GUI based MegaX prototype option. The phylogeny parameters were set to Maximum likelihood, GTR (G+I) etc.
The command that I am running is:
megacc -a analysisFile.mao -d sequences.fas
The error that I get is:
Message = at least 3 sequences are needed for ML analysis
Could anyone help troubleshoot this problem?
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I'm having the same issue, from what I can see it looks like MEGA is only analyzing the 1st sequence no-matter-what format is used .mas .meg .fas. Did you ever figure it out?
i am facing the same issue, too..... did you figure it out now?