Tutorial: raw count data- normalization
I got raw count data,which would consist of 32 samples and 20000 genes.
With this data, I have to normalize this.
first step; normalized gene expression values per sample.
second step: gene-wise normalization
Question 1: What is gene-wise normalization?
Question 2: how can I do this two step?
Question 3: In the first step, do I have to TMM normalization?
I'm a beginner, but no one can help me in my workplace. Please help me.
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