enzyme constants online

Question

[cbasrey]

Hi All,

I am trying to compare different hydrolases from the genus Enterobacter. I was trying to get from online databases constants like Km, Kcat, thermal stability, etc. but I have failed to do so. Can anyone help?

Answer 1

[Elisabeth Gasteiger]

UniProtKB/Swiss-Prot has an annotation topic called "Biophysicochemical properties": http://www.uniprot.org/help/biophysicochemical_properties

Here is a query returning entries from Enterobacter that have such annotation (currently 11 proteins):

http://www.uniprot.org/uniprot/?query=taxonomy%3Aenterobacter%20annotation%3A(type%3Abiophysicochemical_properties)&sort=score&columns=id%2Centry%20name%2Creviewed%2Cprotein%20names%2Cgenes%2Corganism%2Ccomment(PH%20DEPENDENCE)%2Ccomment(REDOX%20POTENTIAL)%2Ccomment(KINETICS)%2Ccomment(TEMPERATURE%20DEPENDENCE)