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Library size factor

Hi all,

Can I get brief explanation why EdgeR and EBseq tools require library size factor for analysis?

Thanks, HJ

rna-seq library size factor
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updated by h.mon h.mon • written by mhyunjunkang • mhyunjunkang

1 answer

Very brief: because of differences in sequencing depth between samples, which need to be take into account.

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written by h.mon h.mon

Thank you for the hint. Does that mean that library size factor is the normalization factor?

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written by mhyunjunkang • mhyunjunkang

Thats one of the normalization factor

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written by lakhujanivijay lakhujanivijay

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