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I'd recommend you get the Biostar Handbook, as it is a complete guide to learning various aspects of bioinformatics; especially relevant to you would be the recently added section on RNA-seq.
Thank you for your reply.
A paper published in BMC Bioinformatics "Evaluation of methods for differential expression analysis on multi-group RNA-seq count data" mentioned: for data without replicates, the DEGES-based pipeline with DESeq2 can be recommended.
Thus, I really confused about which software has the best performance: edggeR, GFOLD or DESeq2?
Thank you!
Quick question. You said:
This post of yours from 3 years ago is on RNA seq: differenrially expressed mRNA pvalue FRD
Those two statements contradict each other. Which of these is accurate please?