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Assessing differentially expressed proteins between two samples

Hi All, My aim is to assess what differentially expressed proteins are detected between two different samples. Each sample is analysed in triplicate therefore I have three replicates for each sample. My question is whether three replicates for each sample are enough to apply t-test to assess what proteins are significantly different between the two samples.

Thanks a lot

differential proteomics analysis
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written by e.scollo • e.scollo

Are you dealing with high-throughoutput data? If so, maybe you should consider using a more robust approach.

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written by h.mon h.mon

Hi Thanks for your reply. With my methodology I can detect approximately 450-500 proteins in each sample. I was planning to use t-test to assess the significance of the differentially expressed proteins. Hope this answer your question

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written by e.scollo • e.scollo

3 replicates for each test should be enough.

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written by Hussain Ather Hussain Ather

Hi Thanks a lot for your reply. Would you use a t-test to assess the significance of the differentially expressed proteins?

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written by e.scollo • e.scollo

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