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fastq Files processing

how to create a fastq file ? or how can I found specific gene inside a fastq file has the whole human genome?

fastqfiles dna-seq fastq fasta

Have you done any research (cough Google cough) on your part? Can you tell us what you looked for and what you found?

I see a history of asking questions without any effort on your part. Please add details on what you've tried or we will have to close the question.

briefly, I have a fastq file include a sequence of one patient, every sequence line that has an id in the fie is consist of 151 characters (A, C, T, G), and I have to extract a specific complete gene sequence from the file!!!!

You can't extract sequence from this file unless you do one of the two following things.

  1. Align your fastq file to a reference sequence. Then use samtools consensus method described here.
  2. de novo assemble you reads (if they are only for that gene region) and then extract a consensus from that assembly.

how to create a fastq file ?

Either by sequencing or creating one using simulation software. File format information here.

Hello emadelhewihy!

Your question is very sparse in details. Please edit your question and add details on why you wish to do what you want to do (so we can address any XY problems) as well as what you've tried. Once you add these details, I'll reopen the question.

For this reason we have closed your question. This allows us to keep the site focused on the topics that the community can help with.

If you disagree please tell us why in a reply below, we'll be happy to talk about it.

Cheers!

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