Entering edit mode
6.0 years ago
willnotburn
▴
50
I would like to annotate a metagenomic assembly with KEGG using the GhostKOALA service. The assembly was produced with megahit and is in FASTA format. GhostKOALA requires amino acid .faa files as input.
Question: what's the best tool to translate the assembly? I'm thinking to use prodigal to detect CDS features and feed those to GhostKOALA. Is there anything out there that's better/faster/newer? I also have bins, and it would be nice to detect rRNA in those, too.
