This is a test version of Biostars. For the public version, visit https://www.biostars.org.
Base pair order in sequencer vs in fastq

For illumina platform, after the reads bind to the chip and bridge amplified, machine reads each read from top to bottom. I am wondering how does this top-bottom order transfer to fastq files. Does it become left to right? In anther word, (assuming index sequence shows in the naming line of fastq, not with the read.) the adaptor that is close to the bottom of chip, will be at the right end of each read (assume this adaptor gets sequenced).

Thank you very much.

sequencing
• 2,723 views • link
updated by finswimmer finswimmer • written by DVA DVA

1 answer

Hello DVA,

yes, "top-to-bottom" becomes "left-to-right". The sequencing direction is always 5' to 3' and that's what you have also in your fastq files. You can also say, the first base you sequence is also the first base in your fastq file.

Here is also a nice picture about the sequence process.

fin swimmer

• 0 views • link
written by finswimmer finswimmer

Thank you for the confirmation!

• 0 views • link
written by DVA DVA

Log in to answer this question.

More posts like this