Just hope to find a primer for sex identification to my samples. What's most acknowledge primer for human sex identification now? SRY or what?
This is what I googled, and not sure whether which is the most acknowledge primer, especially for forensic science
Choice A:
SRY-150F, 5'-AGCAGTCAGGGAGGCAGATCA-3' SRY-245R, 5′-CCCCCTAGTACCCTGACAATGTATT-3′
chrY:2787769-2787864 96bp AGCAGTCAGGGAGGCAGATCA CCCCCTAGTACCCTGACAATGTATT AGCAGTCAGGGAGGCAGATCAgcagggcaagtagtcaacgttactgaatt accatgttttgcttgagaatgAATACATTGTCAGGGTACTAGGGGG
Choice B:
SRY-109F, 5′-TGGCGATTAAGTCAAATTCGC-3′ SRY-245R, 5′-CCCCCTAGTACCCTGACAATGTATT-3′
chrY:2787769-2787905 137bp TGGCGATTAAGTCAAATTCGC CCCCCTAGTACCCTGACAATGTATT TGGCGATTAAGTCAAATTCGCatttttcaggacagcagtagagcagtcag ggaggcagatcagcagggcaagtagtcaacgttactgaattaccatgttt tgcttgagaatgAATACATTGTCAGGGTACTAGGGGG
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This question can only be considered borderline bioinformatics. You may want to post this over at SeqAnswers or Stackexchange.
A google search (for PCR primer for sex identification ) brings up many papers.