I am trying Virmid (mutation calling tool) https://genomebiology.biomedcentral.com/articles/10.1186/gb-2013-14-8-r90 https://sourceforge.net/projects/virmid/

The tools predicts purity of samples and then calls somatic mutation

I ran the tools, it seems to run fine, as it predicts purity, but it gives an error before it produces the SNV list

I tried using two commands,

 java -Xmx4g -jar Virmid.jar -R $assembly -D Tumor.bam  -N Germline.bam
 -t 4 -q 20 -o Out2 -f


 java -Xmx4g -jar Virmid.jar -R $assembly -D Tumor.bam  -N Germline.bam
 -t 4 -q 20 -c1 10 -C1 500 -c2 10 -C2 500 -o Out2 -f

Error message

Exception in thread "main" java.lang.NullPointerException
    at virmid.Param.reportFile(Param.java:174)
    at virmid.Virmid.main(Virmid.java:129)

Similar error has been posted on sourceforge, but no good answer. https://sourceforge.net/p/virmid/discussion/general/thread/fd7aa4df/

Has anyone encountered and solved this issue. Thanks !