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why are the intensities different for two different genes with the same color bead in lincs L1000 data

Why are the intensities different for two different genes with the same color bead in lincs L1000 data? So that the expressions of two genes are Deconvoluted from the fluorescence intensity measured by Luminex scanners. See Figure 1B and reference 1.

Figure 1AB

Ref 1: A Next Generation Connectivity Map, L1000 Platform and the First 1,000,000 Profiles

lincs l1000 luminex

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