Poly(A) tail 5'/3' bias
Is the abundance of Poly(A) tail (A^25), in sample isolated using poly-A mRNA, potentially caused by a 3' bias in the isolation ?
Could using uneven specimen cause bias in analysis (even when filtered)?
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GenoMax
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biostar.anon •
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You need to be a bit more specific - I'm not sure what you are asking...?
I am asking if a overabundance in poly(A) sequences (>1%) in RNA-seq data of a sample could be caused by RNA break during isolation (thus resulting in a 3' bias).
I found a specimen file with an overabundance of A* sequences in fastqc compared all other specimens.
Second, even when filtered for poly(A), tail would such a bias affect DE analysis ?