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velvetg failed during the assembly

Dear all, I have problem during my denovo assembly process,

I have launched velveth for mypaired.fastq containing 600 millions reads in total (length 150) :

velveth out_21 21 -fastq -shortpaired mypaired.fastq

for this steps all were ok

then I launched velvetg on out_21/:

velvetg out_21 -scaffolding no -min_contig_lgth 2000

unfortunately I had this error :

[0.000000] Reading roadmap file out_21/Roadmaps velvetg: Can't calloc 18446744071893837925 Annotations totalling 18446744044658132560 bytes: Cannot allocate memory

have you any ideas about this error? how can i fix it ? thank you in advance .

velvet denovo illumina n50

2 answers

Couple of months back, I also encountered the same error and I didnt get any solution. Similar issue is posted on velvet github issue page but there is no answer to it.

However, when I used older version of velvet (precisely 1.1.07), it solved my problem. So you can also use your velveth result as input for older velvetg version.

great answer. This has saved my run too. thanks a lot

thank you for your answer, I'll try it.

Did it work? I have the same trouble with 1.2.10

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