great answer. This has saved my run too. thanks a lot
Dear all, I have problem during my denovo assembly process,
I have launched velveth for mypaired.fastq containing 600 millions reads in total (length 150) :
velveth out_21 21 -fastq -shortpaired mypaired.fastq
for this steps all were ok
then I launched velvetg on out_21/:
velvetg out_21 -scaffolding no -min_contig_lgth 2000
unfortunately I had this error :
[0.000000] Reading roadmap file out_21/Roadmaps velvetg: Can't calloc 18446744071893837925 Annotations totalling 18446744044658132560 bytes: Cannot allocate memory
have you any ideas about this error? how can i fix it ? thank you in advance .
2 answers
Couple of months back, I also encountered the same error and I didnt get any solution. Similar issue is posted on velvet github issue page but there is no answer to it.
However, when I used older version of velvet (precisely 1.1.07), it solved my problem. So you can also use your velveth result as input for older velvetg version.
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