Hi all,
I've just done normal DNA precipitation in a 50ml falcon (DNA in 10ml H20, 0.1 volume 3M NaOAc, 2.5 volumes 100% Ethanol) and placed it at -80 for 3h. When I came to take it out it was all super thick. Has anyone seen anything like this before? This never happened to me at -20.
My DNA pellet after centrifugation was tiny so I left it at -20 now in hope that whatever did not precipitate at -80 due to the fact that the reaction went weirdly thick, will precipotate at -20 as usual. I'd appreciate any input if anyone has seen something like this or has an idea what might have happened.
1 answer
I usually use ethanol or isopropanol only for precipitation (without water or NaAcOH). Try to add ethanol (total volume) and leave it for a couple of hours in -80 or overnight in -20
Are you sure of the extracted DNA purity? do you have any protein contamination?
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I haven't seen such in my experiments. Btw, I used mostly TE (T10E1) buffer for storage.
sodium acetate of course. I've done the same precipitation hundreds of times at -20. today i wanted to do it at -80 for shorter time and it went strangely thick, which I don't understand.
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