How to compute phred score from a Sanger raw trace ?

Question

[sacha]

Hi,

I wonder how to detect peaks from a sanger raw trace file and compute phred scores for each base called.
For instance here is a vector of 2 peaks :

0
0
0
86
256
484
713
871
901
784
553
279
52
0
10
222
524
826
1033
1085
971
735
455
210
50
0
0

I can easily detect peaks using slope . But I wonder How to assign a phred score for each peaks. Any idea ?

[GenoMax]

See if this helps: http://www.insilicase.com/Web/PhredScores.aspx

[Kevin Blighe]

Also take a look here: https://www.genewiz.com/Public/Resources/Tools-for-Viewing-Sequencing-Data/