Entering edit mode
6.4 years ago
gaber898
•
0
Hey guys, I have RNAseq n=2 data for normal cells and morpholino treated cells which targets a specific protein we believe controls translation of several messengers. Differential expression analysis using DESeq2 produces an MA plot where there is a distinct bias towards lowly expressed genes being downregulated and highly expressed genes being upregulated in the morpholino treated cells. This causes the graph to look rhombus in shape. Has anyone come across this before? Could it be that the cells which are treated with morpholino are dying and degrading their mRNA? I've been looking for similar examples online but can't find any. Does anyone have any ideas? Thanks, Gabriel
