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STAR index generation option --sjdbOverhang for paired-end ?

Hi,

To generate indices for paired-end samples for example with 75 forward and 75 reverse shall I write 75-1 in the option --sjdbOverhang or 150-1 ?

rna-seq

1 answer

74.

The answer is explained here.

Yes, that's correct. It's just read length - 1.

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